Among agriculture workers, pesticide application, especially using manual equipment, seems to be the major determinant

in explaining internal dose. Although the analysis of self-administered questionnaires evidenced difficulties especially related to lack and/or poor quality of reported data, biological monitoring confirms to be a selleck chemical powerful tool in assessing pesticide exposure.”
“Chondroitin sulfate (CS) is used in the treatment of human osteoarthritis as a slow-acting symptomatic drug. For this reason, we performed uptake studies with (TcCS)-Tc-99m using different chondrocyte cultures, as well as cartilage tissue in vitro. For uptake studies, adherent monolayer cultures of human chondrocytes (2.7 x 10(4) cells/well) and (TcCS)-Tc-99m (1 mu Ci) were used. In parallel, we also performed uptake studies with cell suspensions of human chondrocytes at 1 x 10(6) cells/well

incubated with (TcCS)-Tc-99m (5 mu Ci) under identical conditions. Uptake was Studied also in cartilage tissue samples and frozen tissue sections for autoradiography. The uptake was monitored for 10-240 min, every 10-30 min for cell cultures and for cartilage tissue click here up to 72 h. As the commercially available drug Condrosulf (IBSA, Lugano, Switzerland) contains magnesium (Mg) stearate as additive, we investigated the uptake with and without this additive. The washout of the tracer was assessed after the uptake experiments with PBS buffer for different time intervals (10 min-3 h). Tracer uptake in monolayer+/-additives with Torin 2 supplier low number of cells was low. With the use of chondrocytes in Culture suspensions with higher number of cells, a higher uptake of 5.9 +/- 0.65% and 1.0 +/- 0.1% (n=6) was found, with and without additive, respectively. The Saturation was achieved after 100 min. With the use of human rib cartilage, the uptake of (TcCS)-Tc-99m

was continuously increasing with time and was very high with additive amounting to 101.8 +/- 5.2% vs. 53.0 +/- 8.3% (n=6) without after 72 It and showing delayed saturation up to 30 It. Thus, not only the resorption of the drug is enhanced by Mg-stearate, but also the uptake. The washout of the tracer from cartilage after 3 h of uptake amounted to 3.75 +/- 1.5% with additive vs. 13.1 +/- 2.1% without. After 24 h, washout was lower amounting to 1.75 +/- 0.15% vs. 3.25 +/- 0.25%, respectively. The autoradiographic studies paralleled the results of in vitro cartilage tissue uptake. These data show that (TcCS)-Tc-99m accumulates in cartilage tissue, either by acting as a substrate for proteoglycan synthesis or by adsorption to cartilage. (TcCS)-Tc-99m could therefore be a possible agent to target and radioimage osteoarthritis. (C) 2009 Elsevier Inc. All rights reserved.”
“Cancer in the 21st century has become the number one cause of death in developed countries. Although much progress has been made in improving patient survival, tumour relapse is one of the important causes of cancer treatment failure.